Journal: ACS Applied Materials & Interfaces

Article Title: Reducing Small Molecule Adsorption in a PDMS-Based Microphysiological System of the Female Reproductive Tract via Parylene‑C Coating to Improve Mechanistic Studies

doi: 10.1021/acsami.5c20917

Figure Lengend Snippet: Cell viability to evaluate the effects of five different compounds (100 μM of tamoxifen, aspirin, sofosbuvir, and PFOA, 200 μM of celecoxib) on DECs (A) and AECs (B) in 2D 96-well plate experiments after 48 h of exposure. 100 μM of tetraoctylammonium bromide (TAB) served as the positive control, cell-specific culture medium as the negative control, and 0.5% DMSO as the vehicle control. Values are presented as a boxplot (median, interquartile range, max-min), and statistical analysis (compared to negative control) was conducted using the Mann–Whitney U test and Student’s t -test, with statistical significance indicated as * p < 0.05 ( n = 9 for controls, n = 6 for test compounds).

Article Snippet: Specifically, aspirin D4 at 5 μM (18243, Cayman Chemical, Ann Arbor, USA), celecoxib D7 at 1 μM (18248, Cayman Chemical, Ann Arbor, USA), sofosbuvir D6 at 2 μM (HY-15005S1, MedChem Express, Monmouth Junction, USA), repaglinide (tamoxifen) at 1 μM (R9028-50MG, Sigma-Aldrich, St. Louis, USA), and C13 PFOA at 1 μM (MPFOA, Wellington Laboratories, Canada) were used.

Techniques: Positive Control, Negative Control, Control, MANN-WHITNEY

Journal: ACS Applied Materials & Interfaces

Article Title: Reducing Small Molecule Adsorption in a PDMS-Based Microphysiological System of the Female Reproductive Tract via Parylene‑C Coating to Improve Mechanistic Studies

doi: 10.1021/acsami.5c20917

Figure Lengend Snippet: Assessment of tamoxifen (A) and celecoxib (B) bioavailability in coated vs uncoated PDMS-based MPS devices and its impact on the viability of DECs and AECs. Cells were exposed to 100 μM tamoxifen (A) and 50, 100, and 200 μM celecoxib (B) for 48 h in three different culture formats: standard 96-well plates, uncoated PDMS devices, and coated PDMS devices, to evaluate how surface coating influences drug adsorption and cellular response. Values for drug adsorption were normalized to the initial concentration at 0 h and are presented as stacked bars of % stock (mean ± SEM), and statistical analysis (unbound drug concentration [gray bars] in coated versus uncoated devices) was conducted using the Mann–Whitney U test and Student’s t -test, with statistical significance indicated as * p < 0.05 ( n = 3). Values for cellular response are presented as a boxplot (median, interquartile range, max–min), and statistical analysis (coated vs uncoated devices) was conducted using the Mann–Whitney U test and Student’s t -test, with statistical significance indicated as * p < 0.05 and ** p < 0.01 ( n = 3–6).

Article Snippet: Specifically, aspirin D4 at 5 μM (18243, Cayman Chemical, Ann Arbor, USA), celecoxib D7 at 1 μM (18248, Cayman Chemical, Ann Arbor, USA), sofosbuvir D6 at 2 μM (HY-15005S1, MedChem Express, Monmouth Junction, USA), repaglinide (tamoxifen) at 1 μM (R9028-50MG, Sigma-Aldrich, St. Louis, USA), and C13 PFOA at 1 μM (MPFOA, Wellington Laboratories, Canada) were used.

Techniques: Adsorption, Concentration Assay, MANN-WHITNEY